Optimised oligonucleotide substrates to assay XPF-ERCC1 nuclease activity for the discovery of DNA repair inhibitors.
Thomas AM., Brolih S., McGouran JF., El-Sagheer AH., Ptchelkine D., Jones M., McDonald NQ., McHugh PJ., Brown T.
We report the design and optimisation of novel oligonucleotide substrates for a sensitive fluorescence assay for high-throughput screening and functional studies of the DNA repair enzyme, XPF-ERCC1, with a view to accelerating inhibitor and drug discovery.