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Evidence is provided for expression and a functional role for phosphodiesterase type V (PDE-V) in the rat isolated small mesenteric artery. The reverse transcription polymerase chain reaction (RT--PCR) demonstrated mRNA for PDE-V, while Western blotting and immunocytochemical studies showed corresponding protein expression. Smooth muscle relaxation to the nitric oxide donor, diethylamine NONOate (DEA NONOate; 1 nM - 10 microM; pEC(50)=6.7+/-0.3) was potentiated significantly by the specific inhibitor of PDE-V, 4-[[3,4-(methylenedioxy)benzyl]amino]-6-chloroquinazoline (MBCQ; 1 microM; pEC(50)=10.5+/-0.04). These data show that PDE-V is expressed in both the smooth muscle and endothelial cells of a resistance artery, and the enzyme can significantly influence nitric oxide-evoked vasorelaxation.

Original publication




Journal article


Br J Pharmacol

Publication Date





13 - 17


3',5'-Cyclic-GMP Phosphodiesterases, Animals, Arteries, Blotting, Western, Cyclic Nucleotide Phosphodiesterases, Type 5, Immunohistochemistry, In Vitro Techniques, Male, Mesenteric Arteries, Muscle Relaxation, Muscle, Smooth, Vascular, Nitric Oxide Donors, Phosphoric Diester Hydrolases, Precipitin Tests, RNA, Messenger, Rats, Rats, Sprague-Dawley, Reverse Transcriptase Polymerase Chain Reaction, Vascular Resistance