Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Evidence is provided for expression and a functional role for phosphodiesterase type V (PDE-V) in the rat isolated small mesenteric artery. The reverse transcription polymerase chain reaction (RT--PCR) demonstrated mRNA for PDE-V, while Western blotting and immunocytochemical studies showed corresponding protein expression. Smooth muscle relaxation to the nitric oxide donor, diethylamine NONOate (DEA NONOate; 1 nM - 10 microM; pEC(50)=6.7+/-0.3) was potentiated significantly by the specific inhibitor of PDE-V, 4-[[3,4-(methylenedioxy)benzyl]amino]-6-chloroquinazoline (MBCQ; 1 microM; pEC(50)=10.5+/-0.04). These data show that PDE-V is expressed in both the smooth muscle and endothelial cells of a resistance artery, and the enzyme can significantly influence nitric oxide-evoked vasorelaxation.

Original publication

DOI

10.1038/sj.bjp.0703831

Type

Journal article

Journal

Br J Pharmacol

Publication Date

01/2001

Volume

132

Pages

13 - 17

Keywords

3',5'-Cyclic-GMP Phosphodiesterases, Animals, Arteries, Blotting, Western, Cyclic Nucleotide Phosphodiesterases, Type 5, Immunohistochemistry, In Vitro Techniques, Male, Mesenteric Arteries, Muscle Relaxation, Muscle, Smooth, Vascular, Nitric Oxide Donors, Phosphoric Diester Hydrolases, Precipitin Tests, RNA, Messenger, Rats, Rats, Sprague-Dawley, Reverse Transcriptase Polymerase Chain Reaction, Vascular Resistance