NAADP influences excitation-contraction coupling by releasing calcium from lysosomes in atrial myocytes
Collins TP., Bayliss R., Churchill GC., Galione A., Terrar DA.
In atrial myocytes, the sarcoplasmic reticulum (SR) has an essential role in regulating the force of contraction as a consequence of its involvement in excitation-contraction coupling (ECC). Nicotinic acid adenine dinucleotide phosphate (NAADP) is a Ca 2+ mobilizing messenger that acts to release Ca 2+ from an acidic store in mammalian cells. The photorelease of NAADP in atrial myocytes increased Ca 2+ transient amplitude with no effect on accompanying action potentials or the L-type Ca 2+ current. NAADP-AM, a cell permeant form of NAADP, increased Ca 2+ spark amplitude and frequency. The effect on Ca 2+ spark frequency could be prevented by bafilomycin A1, a vacuolar H + -ATPase inhibitor, or by disruption of lysosomes by GPN. Bafilomycin prevented staining of acidic stores with LysoTracker red by increasing lysosomal pH. NAADP-AM also produced an increase in the lysosomal pH, as detected by a reduction in LysoSensor green fluorescence. These effects of NAADP were associated with an increase in the amount of caffeine-releasable Ca 2+ in the SR and may be regulated by β-adrenoceptor stimulation with isoprenaline. These observations are consistent with a role for NAADP in regulating ECC in atrial myocytes by releasing Ca 2+ from an acidic store, which enhances SR Ca 2+ release by increasing SR load. © 2011 Elsevier Ltd.