Optogenetic silencing using light-driven ion fluxes permits rapid and effective inhibition of neural activity. Using rodent hippocampal neurons, we found that silencing activity with a chloride pump can increase the probability of synaptically evoked spiking after photoactivation; this did not occur with a proton pump. This effect can be accounted for by changes to the GABA(A) receptor reversal potential and demonstrates an important difference between silencing strategies.
1102 - 1104
Animals, Hippocampus, Ion Pumps, Neural Inhibition, Neurons, Proton Pumps, Rats, Receptors, GABA-A, Synaptic Transmission