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Direct intra-cerebral administration of substances into the brain parenchyma is a common technique used by researchers in neuroscience. However, inflammatory responses to the needle may confound the results obtained following injection of these substances. In this paper we show that the use of a glass micro-needle for intra-cerebral injection reduces mechanical injury, blood-brain barrier breakdown and neutrophil recruitment in response to the injection of vehicle or interleukin-1, compared to using a 26-gauge Hamilton syringe. Therefore, the use of a glass micro-needle to inject substances intra-cerebrally appears to cause minimal injection artefact and should be the method of choice.

Original publication

DOI

10.1016/j.jneuroim.2007.11.009

Type

Journal article

Journal

J Neuroimmunol

Publication Date

02/2008

Volume

194

Pages

27 - 33

Keywords

Animals, Artifacts, Blood-Brain Barrier, Brain, Brain Injuries, Chemotaxis, Leukocyte, Encephalitis, Glass, Injections, Interleukin-1beta, Male, Meninges, Needles, Neutrophils, Rats, Rats, Sprague-Dawley, Recombinant Proteins, Steel, Syringes