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© 2015 Elsevier Inc.Endolysosomes are emerging as key players that generate as well as respond to intracellular Ca2+ signals. The role of Ca2+ in modulating acidic organelle function has long been recognized, but it is now emerging that acidic organelles also act as intracellular Ca2+ stores; they actively sequester Ca2+ in their lumina and release it to the cytosol upon activation of endolysosomal Ca2+ channels. This local Ca2+ signal is crucial for endolysosomal function and/or global Ca2+ signaling. Importantly, defects in endolysosomal Ca2+ are associated with disease. This chapter discusses several complimentary approaches to monitor endolysosomal Ca2+, with particular emphasis on the inherent pitfalls that can plague the unwary.

Original publication




Journal article


Methods in Cell Biology

Publication Date





159 - 195