Imaging approaches to measuring lysosomal calcium
Morgan AJ., Davis LC., Galione A.
© 2015 Elsevier Inc. Endolysosomes are emerging as key players that generate as well as respond to intracellular Ca 2+ signals. The role of Ca 2+ in modulating acidic organelle function has long been recognized, but it is now emerging that acidic organelles also act as intracellular Ca 2+ stores; they actively sequester Ca 2+ in their lumina and release it to the cytosol upon activation of endolysosomal Ca 2+ channels. This local Ca 2+ signal is crucial for endolysosomal function and/or global Ca 2+ signaling. Importantly, defects in endolysosomal Ca 2+ are associated with disease. This chapter discusses several complimentary approaches to monitor endolysosomal Ca 2+ , with particular emphasis on the inherent pitfalls that can plague the unwary.