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© 2015 Elsevier Inc. Endolysosomes are emerging as key players that generate as well as respond to intracellular Ca 2+ signals. The role of Ca 2+ in modulating acidic organelle function has long been recognized, but it is now emerging that acidic organelles also act as intracellular Ca 2+ stores; they actively sequester Ca 2+ in their lumina and release it to the cytosol upon activation of endolysosomal Ca 2+ channels. This local Ca 2+ signal is crucial for endolysosomal function and/or global Ca 2+ signaling. Importantly, defects in endolysosomal Ca 2+ are associated with disease. This chapter discusses several complimentary approaches to monitor endolysosomal Ca 2+ , with particular emphasis on the inherent pitfalls that can plague the unwary.

Original publication

DOI

10.1016/bs.mcb.2014.10.031

Type

Journal article

Journal

Methods in Cell Biology

Publication Date

01/01/2015

Volume

126

Pages

159 - 195