Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Forward genetic screens in Drosophila melanogaster for modifiers of position-effect variegation have revealed the basis of much of our understanding of heterochromatin. We took an analogous approach to identify genes required for epigenetic repression in human cells. A nonlethal forward genetic screen in near-haploid KBM7 cells identified the HUSH (human silencing hub) complex, comprising three poorly characterized proteins, TASOR, MPP8, and periphilin; this complex is absent from Drosophila but is conserved from fish to humans. Loss of HUSH components resulted in decreased H3K9me3 both at endogenous genomic loci and at retroviruses integrated into heterochromatin. Our results suggest that the HUSH complex is recruited to genomic loci rich in H3K9me3, where subsequent recruitment of the methyltransferase SETDB1 is required for further H3K9me3 deposition to maintain transcriptional silencing.

Original publication

DOI

10.1126/science.aaa7227

Type

Journal article

Journal

Science

Publication Date

26/06/2015

Volume

348

Pages

1481 - 1485

Keywords

Animals, Antigens, Neoplasm, Chromosomal Position Effects, Conserved Sequence, Drosophila melanogaster, Evolution, Molecular, Gene Silencing, Genes, Reporter, Genetic Loci, Green Fluorescent Proteins, HeLa Cells, Heterochromatin, Histones, Humans, Immunoprecipitation, Multiprotein Complexes, Nuclear Proteins, Phosphoproteins, Protein Methyltransferases