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To synthesise stabilised mimics of InsP8, the most phosphorylated inositol phosphate signalling molecule in Nature, we replaced its two diphosphate (PP) groups with either phosphonoacetate (PA) or methylenebisphosphonate (PCP) groups. Utility of the PA and PCP analogues was verified by structural and biochemical analyses of their interactions with enzymes of InsP8 metabolism.

Original publication

DOI

10.1039/c5cc05017k

Type

Journal article

Journal

Chem Commun (Camb)

Publication Date

14/08/2015

Volume

51

Pages

12605 - 12608

Keywords

Binding Sites, Catalytic Domain, Diphosphates, Inositol Phosphates, Molecular Dynamics Simulation, Phosphotransferases (Phosphate Group Acceptor)