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Unnatural amino acid (UAA) incorporation by amber codon suppression offers scientists a powerful tool to modify the properties of proteins at will. UAA incorporation has been used for a plethora of fundamental research applications and, more recently, also for the selective modification of therapeutic proteins. In this review most recent developments in Escherichia coli codon expansion and, unnatural amino acid incorporation are discussed together with some remarkable recent developments in improved efficient UAA incorporation. We focus on the generation of proteins that hold promise for future therapeutic applications that would be impossible to obtain without unnatural amino acid incorporation, including the generation of bi-specific antibodies and antibody drug conjugates.

Original publication

DOI

10.3389/fchem.2014.00015

Type

Journal article

Journal

Front Chem

Publication Date

2014

Volume

2

Keywords

Escherichia coli, amber codon suppression, antibody conjugates, chemical protein modifications, release factor 1, therapeutic proteins, unnatural amino acid incorporation