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Cyclic aristeromycin diphosphate ribose, a carbocyclic analogue of cyclic adenosine diphosphate ribose, was synthesised using a chemo-enzymatic route involving activation of aristeromycin 5'-phosphate by diphenyl phosphochloridate. The calcium-releasing properties of this novel analogue were investigated in sea urchin egg homogenates. While cyclic aristeromycin diphosphate ribose has a calcium release profile similar to that of cyclic adenosine diphosphate ribose (EC50 values are 80 nM and 30 nM, respectively), it is degraded significantly more slowly (t1/2 values are 170 min and 15 min, respectively) and may, therefore, be a useful tool to investigate the activities of cyclic adenosine diphosphate ribose.

Type

Journal article

Journal

FEBS Lett

Publication Date

05/02/1996

Volume

379

Pages

227 - 230

Keywords

Adenosine, Adenosine Diphosphate Ribose, Animals, Calcium, Cyclic ADP-Ribose, Hydrolysis, Ovum, Phosphorylation, Sea Urchins