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Whether the same or distinct steroid sulphatases (STS) are involved in the hydrolysis of alkyl and aryl steroid sulphates remains controversial. We have examined the ability of a placental steroid sulphatase to hydrolyse oestrone sulphate and/or dehydroepiandrosterone sulphate (DHA-S) by expressing the enzyme in COS-1 cells. Using either intact cells or broken cell preparations, the expressed sulphatase was found to hydrolyse both oestrone sulphate and DHA-S. The catalysis of oestrone sulphate and DHA-S by the expressed sulphatase was almost completely abolished by the steroid sulphatase inhibitor, oestrone-3-O-sulphamate. It is concluded that both alkyl and aryl steroid sulphates can be hydrolysed by the same steroid sulphatase.

Type

Journal article

Journal

J Steroid Biochem Mol Biol

Publication Date

07/1994

Volume

50

Pages

101 - 104

Keywords

Animals, Arylsulfatases, Catalysis, Cell Line, Cercopithecus aethiops, Dehydroepiandrosterone, Dehydroepiandrosterone Sulfate, Estrogens, Conjugated (USP), Estrone, Female, Humans, Hydrolysis, Placenta, Steryl-Sulfatase, Transfection