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D-[35S(U)]myo-inositol 1,4,5-trisphosphorothioate [( 35S]InsPS3), a synthetic, metabolically stable analogue of inositol 1,4,5-trisphosphate (InsP3), binds with high affinity (Kd 58.6 +/- 9.1 nM) to rat cerebellar membranes revealing a high density of specific binding sites (Bmax 21.5 +/- 2.1 pmol/mg of protein). Comparison with [3H]InsP3 binding reveals a higher density of sites labelled by [35S]InsPS3 and complex competition curves for displacement of specific [35S]InsPS3 by InsP3. The results suggest that [35S]InsPS3 labels two sites in rat cerebellar membranes with equal affinity: the InsP3 receptor and a site that displays low affinity for InsP3.

Type

Journal article

Journal

FEBS Lett

Publication Date

09/04/1991

Volume

281

Pages

101 - 104

Keywords

Animals, Binding, Competitive, Calcium Channels, Cell Membrane, Cerebellum, Inositol 1,4,5-Trisphosphate, Inositol 1,4,5-Trisphosphate Receptors, Inositol Phosphates, Kinetics, Ligands, Organothiophosphorus Compounds, Rats, Receptors, Cell Surface, Receptors, Cytoplasmic and Nuclear, Sulfur Radioisotopes