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Mung-bean (Phaseolus aureus) nuclease has been found to cleave the Sp diastereoisomer of 5'-O-thymidyl 3'-O-(2'-deoxyadenosyl)phosphorothioate, (Sp)-d[Ap(S)T], in 18O-labelled water with inversion of configuration at phosphorus to give (Sp)-thymidine 5'-[16O, 18O]phosphorothioate, the stereochemistry of which was deduced by methylation to (Rp,Sp)-thymidine 5'-S-methyl-O-methyl-[16O,18O]phosphorothioate and 31P-n.m.r. analysis. This result is consistent with a mechanism involving a direct 'in-line' attack of water on DNA for the nuclease-catalysed reaction without the involvement of a covalent nucleotidylated-enzyme intermediate.

Type

Journal article

Journal

Biochem J

Publication Date

01/02/1987

Volume

241

Pages

827 - 833

Keywords

Chromatography, High Pressure Liquid, DNA, Endonucleases, Hydrolysis, Magnetic Resonance Spectroscopy, Methylation, Models, Chemical, Nucleic Acid Conformation, Oligodeoxyribonucleotides, Single-Strand Specific DNA and RNA Endonucleases, Stereoisomerism, Thionucleotides