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We developed retrograde, transsynaptic pseudorabies viruses (PRVs) with genetically encoded activity sensors that optically report the activity of connected neurons among spatially intermingled neurons in the brain. Next we engineered PRVs to express two differentially colored fluorescent proteins in a time-shifted manner to define a time period early after infection to investigate neural activity. Finally we used multiple-colored PRVs to differentiate and dissect the complex architecture of brain regions.

Original publication

DOI

10.1038/nmeth.1292

Type

Journal article

Journal

Nat methods

Publication Date

02/2009

Volume

6

Pages

127 - 130

Keywords

Animals, Biosensing Techniques, Brain, Green Fluorescent Proteins, Herpesvirus 1, Suid, Luminescent Proteins, Mice, Neurons, Synaptic Transmission, Time Factors, Visual Pathways