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A synthetic connexin-mimetic peptide (Gap 27 peptide) was used to evaluate the contribution of gap junctional communication to smooth muscle responses mediated by the endothelium-dependent agonist acetylcholine (ACh) in rabbit mesenteric arteries. Hyperpolarizations and relaxations to 0.1 and 1 microM ACh observed in the presence of nitric oxide synthase and cyclooxygenase inhibition were markedly attenuated by the peptide at a concentration of 300 microM, whereas the hyperpolarizing response to levcromakalim, a KATP channel opener, was unaffected. The peptide also attenuated intercellular transfer of Lucifer yellow in confluent cultures of COS-7 cells, thus confirming its ability to modulate the permeability of gap junctions. The findings demonstrate that heterocellular gap junctional communication contributes to NO- and prostanoid-independent mechanisms of vasorelaxation that are widely attributed to an endothelium-derived hyperpolarizing factor.

Original publication

DOI

10.1006/bbrc.1998.9877

Type

Journal article

Journal

Biochem biophys res commun

Publication Date

08/01/1999

Volume

254

Pages

27 - 31

Keywords

Animals, COS Cells, Cell Communication, Connexins, Endothelium, Vascular, Gap Junctions, Membrane Potentials, Muscle, Smooth, Vascular, Peptides, Rabbits