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- Galione Group | Organellar Ca²⁺ Channels & Signals Research Group
BSc (Hons), PhD
My research focuses on how cells communicate with one another and the underlying molecular signalling pathways. In particular, how the levels of the intracellular messenger, Ca2+, are controlled by extracellular stimuli in both time and space.
Ca2+ signalling is implicated in myriad cellular events, from fast processes such as neurotransmission to slow processes such as gene expression. When Ca2+ signalling goes wrong, disease ensues. Moreover, Ca2+ signalling pathways can be subverted by infectious agents to their own ends. Therefore, drugs that affect Ca2+ signals may therefore correct diseases or reduce infection.
My current work examines the regulation of Ca2+ release from intracellular Ca2+-storing organelles – mainly endo-lysosomes and the endoplasmic reticulum (ER) – via the second messengers NAADP and IP3. I focus on the bidirectional cross-talk between these two organelles using a combination of molecular biology and single-cell fluorescence microscopy.
Ca2+ dialogue between acidic vesicles and ER.
Morgan AJ., (2016), Biochem Soc Trans, 44, 546 - 553
Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) and Endolysosomal Two-pore Channels Modulate Membrane Excitability and Stimulus-Secretion Coupling in Mouse Pancreatic β Cells.
Arredouani A. et al, (2015), J Biol Chem, 290, 21376 - 21392
Expression of Ca²⁺-permeable two-pore channels rescues NAADP signalling in TPC-deficient cells.
Ruas M. et al, (2015), EMBO J, 34, 1743 - 1758
TPC: the NAADP discovery channel?
Morgan AJ. et al, (2015), Biochem Soc Trans, 43, 384 - 389
Imaging approaches to measuring lysosomal calcium
Morgan AJ. et al, (2015), Methods in Cell Biology, 126, 159 - 195